Microbiology Scientist introduction 1: ZHAO-QING LUO

dearhang

本帖最后由 细菌耐药 于 2017-2-3 01:30 编辑

EducationM.S. Beijing Agricultural University, 1994 Awards and RecognitionsPurdue College of Science Research Award (2015) Other Activities

细菌耐药

Selected Publications:
Qiu J, Sheedlo MJ, Yu K, Tan Y, Nakayasu ES, Das C, Liu X, Luo ZQ. 2016. Ubiquitination independent of E1 and E2 enzymes by bacterial effectors. Nature. 533:120-124. PMID: 27049943 (Highlighted and commented by Nature and Nature Review Microbiology).
Sheedlo MJ†, Qiu J†, Tan Y†, Paul LN, Luo ZQ, Das C. 2015. Structural basis of substrate recognition by a bacterial deubiquitinase important for dynamics of phagosome ubiquitination. Proc. Natl. Acad. Sci. USA. 112(49):15090-5. †, equal contribution authors. PMID: 26598703
Zhu W.†, Tao L.†, Quick M.L,. Joyce J.A., Qu J.M., Luo Z.-Q. 2015. Sensing cytosolic RpsL by macrophages induces lysosomal cell death and termination of bacterial infection. PLoS Pathogens. 11(3):e1004704. doi: 10.1371/journal.ppat.1004704. eCollection 2015 Mar. †,equal contribution authors. PMID: 25738962
Hsu F†., Luo X†., Qiu J†., Teng Y.B., Jin J., Smolka M.B., Luo Z.-Q*., Mao Y*. 2014. The Legionella effector SidC defines a unique family of ubiquitin ligases important for bacterial phagosomal remodeling. Proc. Natl. Acad. Sci. USA. 111(29):10538-43. doi: 10.1073/pnas.1402605111. Epub 2014 Jul 8. †,equal contribution authors. PMID: 25006264
Hsu F., Zhu W., Brennan L., Tao L, Luo ZQ, Mao Y. 2012. Structural basis for substrate recognition by a unique Legionella phosphoinositide phosphatase. Proc. Natl. Acad. Sci. USA.  109:13567-72. PMID:23150405
Tan Y. Arnold A. J. and Luo Z.-Q. 2011. Legionella pneumophila regulates the small GTPase Rab1 activity by reversible phosphorylcholination. Proc. Natl. Acad. Sci. USA. 108:21212-7 PMID: 22158903
Tan Y. and Luo Z.-Q. 2011. Legionella pneumophila SidD is a deAMPylase that modifies Rab1. Nature. 475:506-509. PMID: 21734656.
Fontana M. F., Banga S., Barry K. C., Shen X., Tan, Y., Luo Z.-Q*. and Vance E.V*.  2011. Secreted bacterial effectors that inhibit host protein synthesis are critical for induction of the innate immune response to virulent Legionella pneumophila.  PLoS Pathogens. 7: e1001289. PMID: 21390206
Xu L†., Shen X†., Bryan A., Banga S., Swanson M. and Luo ZQ. 2010. Inhibition of host vacuolar H+-ATPase activity by a Legionella pneumophila effector PLoS Pathogens 19;6(3):e1000822. †, equal contribution authors, PMID: 20333253
Liu Y., Gao P. Banga S. and Luo Z.-Q.  2008. An in vivo gene deletion system for determining temporal requirement of bacterial virulence factors. Proc. Natl. Acad. Sci USA. 105:9385-90. (cited by Faculty 1000). PMID: 18599442
Banga, S., Gao, P., Shen X., Fiscus V., Zong W-X., Chen L. and Luo Z.-Q. 2007. Legionellapneumophila inhibits macrophage apoptosis by targeting pro-death members of the Bcl2 protein family.Proc. Natl. Acad. Sci. USA. 104: 5121-5126. PMID: 17360363

细菌耐药

I just interested in why can he published so many Nature, PLos pathogen, and PNAS papers? Do you want to know?

细菌耐药

I found more information about him as followed.

His laboratory is interested in understanding the cellular and molecular mechanisms that allow microbial pathogens to survive and multiply within the hostile host cells. We use Legionella pneumophila, the causative agent of Legionnaires’ disease as a model organism. This bacterium is a facultative intracellular pathogen capable of growing in a vacuole within macrophages as well as fresh water amoebae. After uptake, the Legionella-containing vacuole (LCV) in its early phase evades fusion with the lysosomal network and later is transformed into a compartment with characteristics of rough endoplasmic reticulum. Essential to the biogenesis of this replicative vacuole is the Dot/Icm Type IV protein secretion system that injects a large number of effector proteins into target host cells. These effectors modulates a variety of host cellular processes, such as remodeling of phagosomal membrane, vesicular trafficking, cell death, stress response thus allowing the establishment of the replicative compartment that supports bacterial growth. Our current focus is to analyze biochemical and cell biological activities conferred by these proteins and their roles in promoting the unique trafficking of the Legionella-containing vacuole in phagocytic cells. In particular, we are interested in identification of host proteins whose activities are modulated by substrates of the Dot/Icm system and the roles of such modulation in intracellular bacterial growth.

Caption for images found in the header: Translocation of effectors into host cell by L. pneumophila in early phase of infection . Host nucleus and bacterium were labeled in red and the effector SidC was labeled in green (Left image). Note the diffusion of SidC from the bacterial phagosome. B. Formation of a large replicative vacuole by L. pneumophila in late phase of infection (Right image). In both cases, note the perinuclear localization of the vacuole.

细菌耐药

members
By using a variety of multidisciplinary methods such as bacterial genetics, cell biology and biochemistry, our studies focus on the mechanisms of how L. pneumophila modulates two cellular processes to promote its intracellular growth. The first is the apoptotic pathways. Our study showed that the bacterium inhibits apoptosis of host cells by interacting with pro- death members of the Bcl2 protein family. The second line of research in this direction is the identification of bacterial factors responsible for the induction of host anti-apoptotic genes. On the other hand, we have identified several bacterial proteins that exert toxic effects to host cells and we are characterizing the biochemical mechanisms underlying such toxicity.

The second focus of our study is the mechanisms of the biogenesis of bacterial vacuoles, particularly those underlying the recruitment of membrane materials from the endoplasmic reticulum (ER) and the inhibition of phagosome acidification by the bacterium. Our recent progress indicated that the Dot/Icm transporter substrate SidJ is required for efficient recruitment of ER proteins to the bacterial phagosome. The current goals are the elucidation of the biochemical activity of this protein and how it coordinates with other effectors to recruit ER materials.

细菌耐药

A new strategy to disrupt the host cytoskeleton by the pathogen Legionella pneumophila
01-30-2017

Title: A new strategy to disrupt the host cytoskeleton by the pathogen Legionella pneumophila

Description of the finding: A new study published in the journal PLOS Pathogens by the Luo lab reveals that the bacterial pathogen Legionella pneumophila attacks the host cell by cleaving actin, an essential component of the cytoskeleton of eukaryotic cells important for their shape and other important cellular processes. The digestion of actin is performed by a protein called RavK, one of the many virulence factors the bacterium uses to counteract the pathogen killing strategy of the host. Cleaved actin lost the ability to form polymers, which is important for its activity. Understanding the mechanism of bacterial virulence is important for the development of novel anti-infective agents and for better appreciation of host cell biology.

Yao Liu, a graduate student in the Luo lab is the first author of the paper; former graduate students Wenhan Zhu and Yunhao Tan, Ernesto S. Nakayasu at Pacific Northwest National Laboratory, Richland, WA and Christopher J. Staiger contributed to the study.

Cleavage by RavK inhibits actin polymerization. A. Cleavage by RavK inhibited actin polymerization in actin sedimentation assay.  After treatment with RavK or its mutant, actin polymerization was allowed to proceed. B. Quantification of the percentage of polymerized actin versus total actin. C. Cleavage by RavK inhibited actin polymerization in a kinetic assembly assay. D. Actin used in pyrene-labeled actin polymerization assay. Groups: i, actin treated with RavK; ii, actin treated with RavKH95A; iii, actin without treatment. Lanes: I, input; P, pellet; S, supernatant.

Link to the paper: http://journals.plos.org/plospat ... ournal.ppat.1006186















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chenqiong

细菌耐药 老师都是英文资料啊！看不懂啊。

明玥

本帖最后由 明玥 于 2017-2-3 07:09 编辑

这篇是介绍微生物专家的对吗？考验英文水平的

lyluoxiuhua

细菌耐药老师如果把英文资料翻译过来分享给我们就好了！

茉莉香

可惜英文基础差，看不懂。要是有翻译就好了。谢谢